Nano biotechnology is the multidisciplinary subject which combines engineering principles and molecular biology. Nano biotechnology has the potentiality to create biological and biochemical materials and devices at molecular and atomic levels. It presents new class of multifunctional systems and devices for biological analysis with better sensitivity and much specificity. Nano biotechnology subsumes the application of the tools and processes of nanotechnology to control biological systems. The Nano biotechnology includes new techniques such as 3D imagining live cells, real-time imaging, and single molecule imaging bio analytical microarrays and biosensors and microfluidic devices. This discipline helps to indicate the subsume of biological research with various fields of nanotechnology. Concepts that are enhanced through Nano biology comprises: Nano devices (such as biological machines), nanoparticles, and Nano scale phenomena that available within the discipline of nanotechnology. This technical approach to biology allows scientists to envisage and create systems that can be used for biological research. Biologically inspired nanotechnology uses biological systems as the encourisation for technologies not yet created. However, as with nanotechnology and biotechnology, bio nanotechnology does have many potential ethical issues associated with it.